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Chování vodních organismů a kontaminace vodních toků? nechtěná léčba deprese a nespavosti u raků
 MAREŠ, Josef
This bachelor thesis evaluates the possible effects of pharmaceutically active compounds on non-target fresh water organisms and the risks connected with them. As model organism was used parthenogenetically reproducing non-native marbled crayfish, which were exposed to a mix of five commonly used drugs and one illicit drug - tramadol, citalopram, sertraline, venlafaxine, oxazepam and methamphetamine. The concentration was 1 microgram.l-1 for each of the tested compounds. The main part of the experiment was based on monitoring the behaviour of the exposed and control group of crayfish in arenas with or without shelter. Prior experiment crayfish were exposed for three-weeks to the mixture of compounds mentioned above. The results were analysed in the programme EthoVisionXT and later also statistically evaluated. In the system with available shelters the observed parameters (distance moved and activity) showed significant differences, where the exposed group walked significantly larger distances and was considerably more active than the control group. Exposed group also spent significantly more time outside the shelter. The results in the system without shelter were almost the opposite. The distance moved, velocity and activity were significantly different. The exposed crayfish showed considerably lower activity, velocity and moved shorter distances. The results of this experiment prove that the mixture of monitored compounds can have a significant impact on the behaviour of non-target fresh water organisms. The compounds had a softening effect and can affect sensory receptors of the exposed crayfish, which can have a significant impact on the populations in open waters.
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